Figure 2
From: P[8] and P[4] Rotavirus Infection Associated with Secretor Phenotypes Among Children in South China
Production of the GST-VP8* fusion proteins and their saliva-based HBGA bindings.
(A) Analysis of the GST-VP8* fusion proteins of P[8] and P[4] rotaviruses by SDS-PAGE with bovine serum albumin (BSA) as standards on the same gels for protein quantitation. Lane 1–3: elution fraction 1 to 3 of the GST-VP8* fusion protein of P[8] rotavirus; lane 4: elution 1 of the GST-VP8* fusion protein of P[4] rotavirus; lane 5–8: BSA standard protein at amounts of 8, 4, 2, 1 μg, respectively; lane 9: VP8* protein of P[8] rotavirus; lane 10: VP8* protein of P[4] rotavirus; lane 11–12: GST elution; lane 13–16: BSA standard protein at amounts of 4, 3, 2, 1 μg, respectively; M: protein marker. (B,C) Eight boiled saliva samples were coated onto 96-well plates and then incubated with the GST-VP8* fusion proteins of P[8] (B) or P[4] (C) rotavirus. The protein was tested in a series of 3-fold dilutions (15 μg/ml, 5μg/ml and 1.7 μg/ml) by enzyme-linked immunosorbent assay (ELISA). (D–G) The binding activities of the GST-VP8* fusion proteins of P[8] (D,E) or P[4] (F,G) rotavirus at 10 μg/ml to 85 saliva samples, among which 75 were from individuals with confirmed rotavirus infection, while 10 were from non-secretor individuals without rotavirus infection. The saliva samples were sorted by blood types (D,F), or by the Leb signals of individual saliva samples (E,G). “A,” “B,” “O,” and “N” represent the type A, B, O and non-secretor saliva, respectively.
