Figure 1

PTEN ameliorates CIA development.

PTEN or mock vector was administered systemically one time in a week into CIA induced mice. Mice were sacrificed ot 9 weeks after first immunization. (A) Clinical scores in CIA induced mice (*P < 0.05, n = 10). (B) The levels of IgG, IgG1, and IgG2a antibodies in each group. Data are presented as mean ± SD of three independent experiments (*P < 0.05, **P < 0.03, n = 10). (C) Joint tissues from CIA mice stained by H&E (original magnification, 40× or 200×, n = 6) or safranin O (original magnification, 200×, n = 6). (D,E) Immunohistochemical detection of IL-6, -21, -17, IL-1β, TNF-α, RANKL, and TRAP after staining in the synovium of CIA mice. (Original magnification, 200×, n = 6). All histological analyses were performed at least 3 times. Representative images are revealed. Data are presented as mean ± SD of three independent experiments (*P < 0.05).