Figure 3

p53 deficiency induces STAT3 activation.

(A,B) The expression levels of p-STAT3 Tyr705 and p-STAT3 Ser727 in splenocytes from WT and p53^−/− mice stimulated by IL-6 (20 ng/ml) for 1 hour were determined by flow cytometry and western blot. (C,D) IL-17A production in splenic CD4⁺ T cells, splenocytes, and draining lymph nodes from WT and p53^−/− mice after incubation for 3 days under Th0 cell conditions (stimulation only with anti-CD3 and anti-CD28 without added cytokines) or Th17 cell–polarizing conditions (stimulation only with anti-CD3 and anti-CD28 with TGF-β and IL-6) were examined by ELISA and flow cytometry. (E) Relative mRNA expression levels of IL-17 and CCL20 in mice splenocytes after incubation for 1 day under Th0 cell conditions and treated with nutlin-3a or bPV(HOpic) were determined by real-time PCR. (F) Relative mRNA expression levels of PTEN in mice splenocytes treated with nutlin-3a or pifithrin-α were determined by real-time PCR. Data are presented as mean ± SD of three independent experiments (**P < 0.03, ***P < 0.01).