Figure 5
(A) SDS–PAGE analysis of reduced glutenin fractions from 12 samples. Glutenin classes were displayed on the left. Precision Plus Protein™ Unstained (Bio-Rad Laboratories, Inc, Hercules CA) was used as molecular marker. HMW: high molecular weight; LMW: low molecular weight; (B) Phylogenetic tree resulting from the analysis of glutenin fractions obtained by SDS-PAGE analysis. Images were processed with Quantity One software (Bio-Rad Laboratories, Inc, Hercules CA) using Ward’s method38 for clustering. For sample references see Table 1. The scale represents the percentage of similarity by the Dice coefficient; (C) SDS–PAGE analysis of reduced gliadin fractions from 12 samples. Gliadin classes were displayed on the left. Precision Plus Protein™ Unstained (Bio-Rad Laboratories, Inc, Hercules CA) was used as molecular marker; (D) Phylogenetic tree resulting from the analysis of gliadin fractions obtained by SDS-PAGE analysis. Image data were processed with Quantity One software (Bio-Rad Laboratories, Inc, Hercules CA) using Ward’s method38 for clustering. For sample references see Table 1. The scale represents the percentage of similarity by the Dice coefficient.
