Figure 5
From: Inhibition of Complement Retards Ankylosing Spondylitis Progression
Complement Activation Enhanced Osteoblastogenesis and Osteoclastic Differentiation in vitro Alizarin Red S staining: Complement activation by zymosan and NHS treatment could result in the osteoblastogenesis of MC3T3-E1 cells.
(B) Statistical analysis of mineral deposition in (A). Data are presented as the mean ± SEM. The significant differences between two groups were determined using the one-tailed Student’s t test for unpaired data. ns, no significance; *P < 0.05; and **P < 0.01. (C) TRAP staining: both RANKL (50 ng/ml) and culture supernatant of MC3T3-E1 cells sustained complement attack by zymosan and NHS could induce the osteoclastic differentiation of RAW264.7 cells. Images are representative of three independent experiments.
