Figure 6
Expression levels of apoptotic proteins and cell cycle regulatory proteins in A549 and CEM cell lines upon treatment with SS28.
A549 and CEM cells were treated with SS28 (5 μM) for 24, 48 and 72 h. Whole cell lysate was prepared and proteins were resolved on a SDS-PAGE and western blotting was performed using specific primary and secondary antibodies. Blots shown are representative blots of two independent experiments with identical results. Actin was used as the loading control. (A) For CEM cells p53, Ku70, Cyclin B1, Cdk6, PARP-1, Caspase 9 and Caspase 3 proteins were evaluated. (B) Quantification of the proteins shown in panel A is represented as bar diagram with error bars. (C) For A549 cells, p73, Ku80, Cdk6, Cyclin B1, Caspase 9 and Caspase 3 proteins were evaluated. (D) Quantification of the respective proteins is shown in bar diagram with error bars.
