Figure 3 | Scientific Reports

Figure 3

From: Deficiency in Toll-interacting protein (Tollip) skews inflamed yet incompetent innate leukocytes in vivo during DSS-induced septic colitis

Figure 3

Neutrophils in Tollip−/− mice accumulated in blood and failed to migration to injured mucosa after exposure to DSS.

(A,B) CCR5 and FPR2 expression on neutrophils (gated on Ly6G + CD11b + ) in blood via flow cytometry. (C) Flow cytometry about neutrophils (CD11b+ Ly6G+) in blood harvested at day 0, day 6, day12 during the process of experimental colitis. (D) Immuno-histological staining (left) of neutrophils (Ly6G+) in the injured mucosa of colon; assessment (right) about neutrophils with stained particles using Image J. (E) Western blot of FPR2 expression on purified neutrophils in blood at day 6. The symbols *,**indicate P < 0.05, 0.01 between the DSS-treated gene-deficient strains compared with DSS-treated WT. The symbols #,##,###indicate P < 0.05, 0.01, 0.001, and &,&&&indicate P < 0.05, 0.001 between the WT control and DSS-treated WT or between Tollip−/− control and DSS-treated Tollip−/− mice, respectively. WT control n = 3; Tollip−/− control n = 3; DSS treated WT (day 6), n = 5; DSS treated Tollip−/− (day 6), n = 5; DSS treated WT (day 12), n = 5; DSS treated Tollip−/− (day 12), n = 4.

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