Figure 4 | Scientific Reports

Figure 4

From: Deficiency in Toll-interacting protein (Tollip) skews inflamed yet incompetent innate leukocytes in vivo during DSS-induced septic colitis

Figure 4

Tollip−/− neutrophils demonstrated an inflamed yet incompetent phenotype in vitro.

(A) Chemotaxis of neutrophils from bone marrow to fMLF (10−5mM). (B) FPR2 expression on neutrophils (gated on Ly6G + CD11b + ) from bone marrow via flow cytometry. (C) H4 expression in neutrophils (gated on Ly6G + CD11b + ). (D) Neutrophil bacteria killing assay. (E) Western blot of purified neutrophils from bone marrow. The symbols *,**indicate P < 0.05, 0.01 between the DSS-treated gene-deficient strains compared with DSS-treated WT. The symbols #,##indicate P < 0.05, 0.01, between the WT control and DSS-treated WT.

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