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Figure 1

From: LC-MS Based Sphingolipidomic Study on A2780 Human Ovarian Cancer Cell Line and its Taxol-resistant Strain

Figure 1

Differentiation of isomeric SPLs by targeted MS/MS.

(a) Two peaks were observed at m/z 620.59 in extracted ion chromatogram of TOF MS. Accurate MS/MS data confirmed these peaks corresponding to Cer (d18:1/22:1) and Cer (18:2/22:0) due to the characteristic fragment at 264.3 and 262.3 respectively. In MRM mode, target ion pairs consist of the same parent ion (620.6) but different daughter ions [(b) 264.3 for Cer (d18:1/22:1) and (c) 262.3 for Cer (18:2/22:0)] were employed for the accurate quantitation.

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