Figure 2
Differentiation of isotopic SPLs by accurate MS together with complete separation.
(a) SM (d18:1/14:0) (tR = 11.190 min) yields precursor ions at m/z 675.5427, 676.5459 and 677.5484. The last one is the [M + 2] isotopic ion which will interfere with the precursor ion of (b) SM (d18:0/14:0) (tR = 11.523 min) at m/z 677.5576. If the chromatograph cannot separate the two SPLs completely, the quantification result of SM (d18:0/14:0) will be artificially high.
