Figure 4

ANGPTL6 expression is elevated in human psoriatic skin.

(a–c) Immunohistochemical staining of serial sections of human skin tissues with antibodies against ANGPTL6, S100A8 and S100A9. Shown are samples from two representative psoriasis patients, including (a) Patient 1 (37-year-old female (Case No. 2 in Table 3)) and (b) Patient 2 (37-year-old female (Case No.14 in Table 3)). (c) Also shown is a sample from a healthy control subject (34-year-old female (Case No. 2 in Table 4)). Magnified images of each dotted box are shown in Supplementary Fig. S5. Scale bar: 100 μm. (d) Representative confocal images from double immunofluorescence staining for AE1AE3 (keratinocyte marker; green) and ANGPTL6 (red, top row); CD68 (macrophage marker; green) and ANGPTL6 (red, second row); CD11c (dendritic cell marker; green) and ANGPTL6 (red, third row); and CD1a (Langerhans cell marker; green) and ANGPTL6 (red, bottom row) of a human psoriatic lesion (56-year-old male (Case No. 10 in Table 3)). Scale bar: 100 μm. Right column shows magnified images of boxed regions in adjacent image. Scale bar: 50 μm.