Figure 2
From: Exome sequencing in the knockin mice generated using the CRISPR/Cas system
Analysis of the 3 InDels identified.
(a) Sequence information of the 3 InDel sites. Yellow highlighted nucleotides, PAM sequence for Ntrk1 target site; gray highlighted nucleotides, putative PAM sequence for Aspa insertion site. Boldface nucleotides, matched bases between the guide sequence and the Aspa sequence. (b) In vitro cleavage assay for the 3 InDel sites. Cleavage templates: PCR products amplified from wild-type B6J mouse genomic DNA including the sites for Ntrk1 target, InDels (Zfp365, Alox12, Aspa) and Ntrk2 genomic region corresponding to the Ntrk1 target.
