Figure 2

CHIP-PCR analyses of H3 and H4 acetylation in the SOD1 promoter of cultured rabbit lenses treated with AA (AAM group) and AA+TSA (AT group).
(A) AA did not change the histone acetylation position at the SOD1 promoter. The acetylated histones were at −1200 and −900 bp in all of the groups (A(1) control group; A(2) AAM group; A(3) AT group). (AA significantly reduced histone acetylation at both −1200 and −900 bp ((B) H3 relative acetylation level; (C) H4 relative acetylation level). TSA effectively prevented histone hypoacetylation induced by AA. *p < 0.05.