Table 3 Efficiency of anti-Cronobacter sakazakii immunoglobulin G conjugation with magnetic nanoparticles.

From: Detection of Cronobacter sakazakii in powdered infant formula using an immunoliposome-based immunomagnetic concentration and separation assay

Antibody

Amount of antibody

Coefficient of variation

Initial antibody

8.35 ± 0.00 mg/ml

—

Free antibody

0.96 ± 0.01 mg/ml

1.88%

Bound antibody

7.39 ± 0.59 mg/ml

1.36%

Conjugation efficiency

88.5%

2.62%

  1. Conjugation efficiency (%) = Bound (initial–free) antibody/initial antibody × 100. The antibody concentration was measured with the Bradford assay. All experiments were conducted three times, and data represent the mean ± standard deviation. The coefficient of variation (%) was determined via intra assay test by running 10 replicates of the experiment in one analytical run.
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