Figure 2 | Scientific Reports

Figure 2

From: Impact of splenic artery ligation after major hepatectomy on liver function, regeneration and viability

Figure 2

Evaluation of cellular viability and death by flow cytometry.

(a) Live cells. (b) Initial apoptosis. (c) Late apoptosis/necrosis. (d) Necrosis. Hepatocytes were extracted at 24, 48, 72 and 120 postoperative hours, from rats submitted to hepatectomy (Hx) or hepatectomy + splenic artery ligation (Hx + Asp). Cell death was detected by annexin V and propidium iodide staining and analysed by flow cytometry. Results are expressed as average of percentage of each type of cells ± standard deviation obtained from six rats in each group. ***p < 0.001; **p < 0.01; *p < 0.05. — Baseline value in a control group without any procedure.

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