Figure 2
dsDNA and CDN bind to the DEAD domain at the same sites.
(A) Pull-down assays of DEAD, using bio-cGG in the presence of unlabeled ISD (left panel), and bio-ISD in the presence of unlabeled cGG (right panel). The final concentrations of unlabeled ISD or cGG are 0, 0.5, 1, 2 or 4 μM. (B) Pull-down assays of DEAD, using bio-ISD (left panel) or bio-cGG (right panel), in the presence of 1 mM unlabeled ATP, ADP or AMPPNP. Gels were run under the same experimental conditions and are shown as cropped gels. Full-length gels with indicated cropping lines are shown in Figure S7.
