Figure 3

Heterologous expression of OsIFL in yeast cells improves their tolerance to salinity and osmotic stress.

Growth curve assay of WT, vector transformed (WT+vector) and pYES2OsIFL transformed (WT+OsIFL), BY4741 S. cerevisiae cells in the presence of (a) 1M NaCl and (b) 1M Mannitol showed vigorous growth of pYES2OsIFL transformed cells in comparison with WT and vector transformed cells. Growth of these strains observed on (c) Solid YPG media; (d) Solid YPG media containing 1M NaCl or (e) 500 mM Mannitol. Initial O.D₆₀₀ ~ 0.3 and subsequent serial dilution (10⁻¹ to 10⁻⁷) used for these assays. Plates were incubated at 30 °C and images were taken after 72 h. WT - untransformed cells (WT), WT+ vector - vector transformed cells and WT+OsIFL - pYES2OsIFL transformed cells.