Table 1 The crack ratio, and crystal area of CLPCs in an orthogonal array L16(45).

From: Preparation of cross-linked hen-egg white lysozyme crystals free of cracks

Experimental run

Factors

Crack ratio

Crystal area

Aa

Bb

Cc

Dd

Ee

1

1

1

1

1

1

0

0.0266

2

1

2

2

2

2

0.0769

0.2164

3

1

3

3

3

3

0.2541

0.1031

4

1

4

4

4

4

0.4472

0.0207

5

2

1

2

3

4

0

0.0324

6

2

2

1

4

3

0.0378

0.2541

7

2

3

4

1

2

0.3399

0.2553

8

2

4

3

2

1

0.3942

0.0289

9

3

1

3

4

2

0

0.0679

10

3

2

4

3

1

0.4148

0.2428

11

3

3

1

2

4

0.1401

0.0195

12

3

4

2

1

3

0.1841

0.0119

13

4

1

4

2

3

0

0.1074

14

4

2

3

1

4

0.3095

0.2468

15

4

3

2

4

1

0.0800

0.0230

16

4

4

1

3

2

0.2271

0.0098

  1. aA represents temperature,
  2. bB represents pH value,
  3. cC represents crystal growth time,
  4. dD represents glutaraldehyde concentration,
  5. eE represents cross-linking time.
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