Table 2 Two-way ANOVA testing the effect of AS and VHSv challenge on transcriptional regulation of IGF-IR genes in rainbow trout at different developmental stages.

From: The complete salmonid IGF-IR gene repertoire and its transcriptional response to disease

Treatment

Gene

P-value treatment

P-value treatment* stage

H transcript level (SD)

Fold change

FF transcript level (SD)

Fold change

3wFF transcript level (SD)

Fold change

AS (n = 4)

IGF-IRa1 1

<0.0001

0.03

66.57 (26.12)

2.39*

53.96 (12.41)

1.44*

53.65 (8.84)

1.30

IGF-IRa2

<0.0001

0.25

32.63 (8.50)

1.93*

29.65 (5.77)

1.52*

33.99 (5.49)

1.23

IGF-IRb

0.09

0.91

24.97 (12.84)

1.38

29.33 (8.34)

1.15

25.70 (3.46)

1.25

VHSv (n = 3 to 5)

IGF-IRa1

0.002

0.01

33.66 (11.20)

1.21

62.00 (11.28)

1.66*

41.86 (5.29)

1.01

IGF-IRa2

0.003

0.02

15.66 (4.99)

0.93

33.42 (6.73)

1.72*

33.72 (4.03)

1.22

IGF-IRb

0.01

N/A

21.09 (3.59)

1.17

35.52 (8.29)

1.39

36.74 (4.72)

1.78*

  1. Transcript levels shown are for infected animals.
  2. 1Data transformed by natural logarithm.
  3. N/A: No treatment*stage interaction tested, as statistics done with Kruskal-Wallis test on treatment effect only.
  4. Fold change: mean transcript levels of treatment/control.
  5. Asterisks (*) indicate significant (P < 0.05) effect of treatment at each stage of development.
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