Table 4 Primers used for qPCR analyses.
From: The complete salmonid IGF-IR gene repertoire and its transcriptional response to disease
Gene | Accession Number | Sense Primer (5′-3′) | Tm (°C) | Antisense Primer (5′-3′) | Tm (°C) | Efficiency (%) | Product Size (bp) |
|---|---|---|---|---|---|---|---|
IGF-IRa1 | XM_014124119 | AAACGGGACTATGACAACGCA | 60 | CGGGTCTCAGGCTCGTCC | 61 | >89 | 308 |
IGF-IRa2 | XM_014149105 | CACGGGACTACGACATCACG | 59 | CGCGTCTCTGGGTCGTCT | 59 | >86 | 307 |
IGF-IRb | XM_014176744 | TTTGGCGTGGCTAACCGTA | 60 | GCCAGCGGAGGAACACAG | 59 | >89 | 331 |
IGF-I 52 | GU933431 | GTGATGTCTTCAAGAGTGCGATGTG | 64 | CGCCCCTGTTGCCGCCGAA | 74 | 99 | 98 |
IGF-II 26 | M95184 | AACACAAGAATGAAGGTCAAGATG | 58 | ACTCCTCCACGATACCACGG | 60 | 94 | 226 |
RpL4 52 | BT057966 | CCTTCAGAAACATCCCTGGTATCAC | 63 | GGGCAGATTGTAGTCTACCTTGAGAG | 62 | >83 | 182 |
RpS13 52 | BT059859 | CCCTCTCAGATCGGTGTGATCC | 63 | TCCTTGTCCTTTCTGTTCCTCTCC | 63 | >90 | 191 |
RpS29 52 | BT043522 | GGGTCATCAGCAGCTCTATTGG | 61 | AGTCCAGCTTAACAAAGCCGATG | 63 | >87 | 167 |
ACTB 53 | AF012125 | TGACCCAGATCATGTTTGAGACC | 61 | CTCGTAGATGGGTACTGTGTGGG | 61 | >89 | 146 |
EF1A 54 | AF321836 | CAAGGATATCCGTCGTGGCA | 62 | ACAGCGAAACGACCAAGAGG | 60 | >90 | 327 |
