Figure 3

Cxcr5 deletion reduces neuronal excitability of DRG neurons.

(A) Examples of membrane potential responses evoked by 1000 ms ramp current injection of 100, 200, and 300 pA for neurons from saline-and CFA-treated wild type (WT) or Cxcr5^−/− mice. (B) Histogram shows an increase in numbers of action potential (AP) of DRG neurons from CFA-treated WT mice. Two-way repeated measures ANOVA (Treatment, F_3,84 = 15.433, P < 0.001; Current, F_2,84 = 123.976, P < 0.001; Interaction, F_6,84 = 3.811, P < 0.05). **P < 0.01, ***P < 0.001, compared to WT-saline. ^##P < 0.01, ^###P < 0.001, compared to WT-CFA, post hoc Bonferroni test. (C) Examples of membrane potential responses evoked by 1000 ms current injection of 100, 200, and 300 pA for the PBS and CXCL13-treated neurons from WT or Cxcr5^−/− mice. (D) Histogram shows an increase in numbers of AP of DRG neurons from WT mice after CXCL13 treatment. Two-way repeated measures ANOVA (Treatment, F_3,114 = 8.564, P < 0.001; Current, F_2,114 = 161.529, P < 0.001; Interaction, F_6,114 = 0.873, P > 0.05). **P < 0.01, ***P < 0.001, compared to WT-PBS. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, compared to WT-CXCL13, post hoc Bonferroni test.