Figure 4
From: Defining cutaneous molecular pathobiology of arsenicals using phenylarsine oxide as a prototype
Effects of 4-PBA and NAC in PAO-induced pathological changes in Ptch1+/−/SKH-1 mouse skin.
Mice were treated topically with either vehicle or PAO (100 μg/mouse) on the dorsal skin area followed by 4-PBA (4 mg/mouse) or NAC (5 mg/mouse) at 5 min post PAO challenge. Mice were euthanized at 8 h and skin samples were collected for histochemical analysis. (A) Mouse skin photographs show topical efficacy of 4-PBA and NAC on PAO-induced skin gross changes. PAO-treated mouse skin show thick, leathery, and wrinkled skin while application of 4-PBA and NAC at 5 min post PAO challenge diminishes these effects. (B) Immunohistochemical staining of ATF4 and CHOP. 4-PBA and NAC effectively reduces PAO-induced expression and nuclear localization of ATF4 and CHOP in epidermal keratinocytes. (C) Histochemical (H&E) analysis of the skin sections. Blue arrows represent mv formation and black arrows represent inflammatory leukocytes infiltrations. (D) Topical efficacy of 4-PBA and NAC on murine skin show effects of 4-PBA and NAC on PAO-induced size (I) and number (II) of micro-vesicants formation in murine skin. Data are expressed as mean ± SEM. ***P < 0.001 when compared to control. ###P < 0.001 when compared to PAO-treated group.
