Figure 3
Scallop hepatopancreas α-L-fucosidase analyses.
(A) Phylogenetic analysis of α-L-fucosidases. The C. farreri (Cf) sequences are highlighted by black triangles in both the genome data and proteomic analyses. Hollow triangles represent sequences present only in the genome data. Fuca: α-L-fucosidase; Cg: Crassostrea gigas; Lg: Lottia gigantean; Ac: Aplysia californica; At: Arabidopsis thaliana. The accession numbers of the protein sequences are provided in Supplementary Table S2. (B) Amino acid sequence alignment of the α-L-fucosidases. Identical residues are shaded in gray. Chlamys farreri (Fuca1–7) and Thermotoga maritime (FucaT, PDB: 1HL8). The two catalytic residues identified in FucaT22 are marked with closed circles and the three residues that form the substrate binding pocket are marked with triangles. The gap described in the text is located in the boxed region. (C) Structure of FucaT. The FucaT structure from Thermotoga maritime (PDB: 1HL8) is illustrated with yellow ribbons and the key residues shown as sticks. The loop adjacent to the substrate binding pocket is highlighted by a dashed line. The image was generated by PyMOL32.
