Figure 4
Analysis of scallop hepatopancreas arylsulfatases.
(A) Phylogenetic analysis of arylsulfatases. The C. farreri (Cf) sequences are highlighted by black triangles in both the genome data and proteomic analyses. Hollow triangles represent sequences present only in the genome data. ARSB: arylsulfatase; Cg: Crassostrea gigas; Lg: Lottia gigantean; Ac: Aplysia californica; Ng: Nannochloropsis gaditana CCMP526. The accession numbers of the protein sequences are provided in Supplementary Table S2. (B) Amino acid sequence alignment of arylsulfatases. C. farreri (ARSB1–4) and human (ARSBH, PDB: 1FSU). Identical residues are shaded in gray. The ten primary active-site residues described in ARSBH are marked with triangles. (C) Structural analysis of ARSBH. The human structure of ARSBH (PDB: 1FSU) illustrated with orange ribbons and with the C-terminal region in blue. The primary active-site residues are shown as sticks, and the catalytic center is highlighted by a dashed circle. The picture was generated by PyMOL32.
