Figure 5
Neuritogenesis and polarity of cultured hippocampal and MGE neurons.
(a,b) Representative micrographs of cultured hippocampal neurons transfected with a GFP-expression vector, after 7 DIV, to examine neuritogenesis in the presence (WT, a) or in the absence (b) of ArhGAP15. Most neurons were morphologically pyramidal. Scale bar is reported in panel a. (c–e) Quantification of the length of the longest neurite in μm (e), of the number of branches (secondary neurites) (f) and the mean number of intersections as a function of distance from the soma (Sholl analysis, in i), comparing neurons from WT (black bars) and ArhGAP15−/− (open bars) brains. (f,g) Quantitative analysis of neuronal polarity, expressed and the number of unipolar, bipolar and multipolar neurons (j) and as the number of primary neurites in multipolar neurons (k), comparing neurons from WT (black bars) and ArhGAP15−/− (open bars) brains. A total of 120 neurons were examined for each genotype. (h,i) Representative micrographs of cultured neurons from dissociated MGEs from wild-type (h) or ArhGAP15−/− E14.5 embryos, after 7 DIV. A large fraction of these neurons were shown to be GAD67 immunoreactive. (j–l) Quantitative analyses on neurite length (j), branching (k) and overally complexity by Sholl analysis (l). A total of 140 neurons were examined for each genotype. *Indicates p < 0.05; ***Indicate p < 0.001.
