Figure 6 | Scientific Reports

Figure 6

From: Disruption of ArhGAP15 results in hyperactive Rac1, affects the architecture and function of hippocampal inhibitory neurons and causes cognitive deficits

Figure 6

Spine density of hippocampal neurons upon downmodulation of ArhGAP15.

(a,b) Representative images of dendritic spines of primary rat hippocampal neurons electroporated with a GFP-expressing vector, and treated with either a control (c) or an anti-ArhGAP15 siRNA (d) oligonucleotide sequence. (c,d) Higher magnification of apical dendrites from c and d, respectively. Scale bar (=1 μm) is reported in panel c. (e–g) Histograms showing the quantification of spine density (e), length (f) and width (g) on the apical dendrites of pyramidal neurons transduced with control (solid bars) or with siRNA for ArhGAP15 (open bars). A total of 40 neurons in three experiments was examined for each genotype. Density is expressed as number of spines per 10 μm of dendrite. A significant reduction of spine density is observed when endogenous ArhGAP15 is silenced, while spine length and width were unchanged. *Indicates p < 0.05; ***Indicate p < 0.001.

Back to article page