Figure 1 | Scientific Reports

Figure 1

From: Intra-islet lesions and lobular variations in β-cell mass expansion in ob/ob mice revealed by 3D imaging of intact pancreas

Figure 1

Islet β-cell distribution in ob/ob and lean control mice between 4 and 52 weeks of age.

(A–J) Iso-surface rendered OPT images of representative splenic lobes of lean control (A–E) and ob/ob (F–J) pancreata. The islet β-cell volumes are reconstructed based on the signal from insulin specific antibody staining (red) and pancreas outline (gray) is based on the signal from tissue autofluorescense. In contrast to lean controls, the expected expansion in BCV is clearly observed in ob/ob pancreata. (K) Graph showing the average total β-cell volume in ob/ob and lean controls illustrating the progressive increase in ob/ob BCV (entire pancreas). (L) Graph showing the average total pancreas volume. (M) Percentage of the total pancreatic volume constituted by insulin positive cells. Data is shown as means ± SEM (n = 5) where *P < 0.05; **P < 0.01 and ***P < 0.001. Scale bar in (J) corresponds to 2 mm in (A–J).

Back to article page