Figure 3
ob/ob β-cell expansion by islet β-cell volume size categories.
(A–J) Iso-surface rendered OPT images of representative splenic lobes of lean control (A–E) and ob/ob (F–J) pancreata between 4 and 52 weeks of age. Individual islet β-cell volumes are reconstructed based on the signal from insulin-specific antibodies and have been pseudo colored to highlight the distribution of small (<1 × 106 μm3 [white]), intermediate (1 × 106 −5 × 106 μm3 [red]), and large (>5 × 106 μm3 [yellow]) islets. (K) Histogram showing the average total β-cell volume constituted by each size category in lean control and ob/ob pancreata respectively. (L) Histogram showing the average total number of islets within each size category in lean control and ob/ob pancreata respectively. Data is shown as means ± SEM (n = 5) where *P < 0.05; **P < 0.01 and ***P < 0.001. Abbreviations: S, small; M, medium; L, large. Scale bar in (J) corresponds to 2 mm in (A–J).
