Figure 4
Cystic lesions are frequently formed in hypertrophic ob/ob islets.
(A) Tomographic OPT section from a representative ob/ob pancreas at 26 weeks of age (splenic lobe). Insulin positive areas are pseudocolored red. (See also Movie S2). (B–D) Iso-surface rendered OPT images of the specimen in (A). The islet β-cell volumes are reconstructed based on the signal from insulin specific antibody staining (B, red) and the outlines of the cystic lesions were manually delineated in the tomographic sections (C, gray). The islet β-cell volumes and the areas occupied by lesions are merged together with the outline of the organ (dark gray) in (D). (E–H) Representative ultramicroscopy images of hypertrophic islets at 26 weeks (duodenal lobe) showing the surface morphology (E,F) and the same islets applying a digital section plane to visualize the internal lesions (G,H) Area marked by a broken line in (E,G) corresponds to high magnification images in (F,H). White arrow in (E) points to a cystic lesion that has generated an opening towards the surface of the islet. Abbreviations; SL, Splenic lobe; DL, Duodenal lobe; Tom. section, Tomographic section. Scale bar in (D) is 506 μm in (A) and 2 mm in (B–D). Scale bar in H is 291 μm in (E,G) and 100 μm in (F,G).
