Figure 5
Effect of intracarotid BHB infusion on mRNA expression of liver enzymes involved in gluconeogenesis, glucose production and glycogen synthesis as well as on liver glycogen content.
(a) PEPCK mRNA expression at 24 h in mice infused with either NaCl or BHB (b) G6Pase mRNA expression at 24 h in mice infused with either NaCl or BHB (c) GlycogenPase mRNA expression at 24 h in mice infused with either NaCl or BHB (d) Liver glycogen content at 24 h in mice infused with either NaCl or BHB. mRNA expression for each enzyme was determined by quantitative reverse transcriptase–PCR at 24 h of NaCl or BHB infusion. Glycogen content was determined by the quantification of the glucose amount produced from glycogen hydrolysis. (e) Liver G6Pase and PEPCK protein expression at 24 h in mice infused with either NaCl or BHB. Upper panels are representative Western blots. Lower panel provides the quantification of protein expression. G6PAse or PEPCK protein levels in BHB groups are expressed as percentage of the expression in the corresponding NaCl-treated group (set at 100%). β-tubulin was used as internal reference. Data represent the mean ± SEM with n = 12 animals per condition and were statistically analyzed with unpaired Student t-test. *p < 0.05 vs. NaCl; **p < 0.01 vs. NaCl. a.u., arbitrary units.
