Figure 4

Visceral total WAT and adipocytes.

Visceral total white adipose tissue (WAT) weight (a,c) and percentage of body weight (b,d) of Mc3r KO, Mc4r KO, DKO and WT controls of 14-week old male and female rats (n = 8 except 4–6 for DKO). Data are shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 vs. WT control (one-way ANOVA test). (e) Representative images of adipocytes of Mc3r KO, Mc4r KO, DKO rats and WT littermates, together with quantitation (magnification ×100 and ×400), from at least 3 animals of each genotype.