Figure 5
From: Effects of Melanocortin 3 and 4 Receptor Deficiency on Energy Homeostasis in Rats
Alteration of liver in MC3R and MC4R deficient rats.
(a) Relative liver weight (as percentage of body weight) of Mc3r KO, Mc4r KO, DKO and WT littermates at 14 weeks (n = 8). (b) Alanine aminotransferase and (c) Aspartate aminotransferase of Mc3r KO, Mc4r KO, DKO and WT littermates (n = 8). Data are shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 vs. WT littermates (one-way ANOVA test). (d,e) Representative image of HE staining and (f,g) Oil Red-O staining of livers from at least 3 animals per group (magnification ×100). Relative areas of vacuolation or Oil Red O staining were quantified using Image-Pro® Plus version 6.0 software.
