Figure 3
From: mTOR is critical for intestinal T-cell homeostasis and resistance to Citrobacter rodentium
Effects of mTORC1 or mTORC2 deficiency on T-cell numbers in the LP compartments of the intestines.
SI-LP and LI-LP single cell preparations from Rptorf/f and Rptorf/f-CD4Cre mice (A–E) or Rictorf/f and Rictorf/f-CD4Cre mice (F–J) were stained and analyzed similarly as in Fig. 2. (A–E) Rptorf/f and Rptorf/f-CD4Cre mice. (A) Representative dot-plots shown CD45 vs TCRβ staining. (B,C) Scatter graphs showing the mean ± SEM of αβT-cell percentages within CD45+ cells (B) and numbers (C,D) CD4 and CD8 T-cell numbers. (E) Percentages of CD4 and CD8 subsets within αβT-cells. (F–J) Rictorf/f and Rictorf/f-CD4Cre mice. Representative dot-plots shown CD45 vs TCRβ staining. (G,H) Scatter graphs showing the mean ± SEM of αβT-cell percentages within CD45+ cells (G) and numbers (H,I) CD4 and CD8 T-cell numbers. (J) Percentages of CD4 and CD8 subsets within αβT-cells. Each circle represents one mouse. Data shown are representative or calculated from at least five experiments. *P < 0.05; **P < 0.01; ***P < 0.001 determined by unpaired two-tailed Student t-test.
