Figure 4

Decreased effector T-cells in mLNs and PPs in the absence of mTOR.

PP and mLN single cell preparations from mTOR^f/f and mTOR^f/f-CD4Cre mice were stained for CD4, CD8, CD44, CD62L and TCRβ followed by flow-cytometric analysis. (A) Scatter graphs showing the mean ± SEM of total T, CD4, and CD8 T-cell numbers in the spleen, pLNs, mLNs, and PPs from mTOR^f/f-CD4Cre and control mice. (B) Percentages of CD4 and CD8 CD44⁻CD62L⁺ naïve, CD44⁺CD62L⁻ effector memory (EM), and CD44⁺CD62L⁺ central memory (CM) T-cells in mLNs and PPs from mTOR^f/f-CD4Cre and control mice. Each circle represents one mouse. Data shown are calculated from at least five experiments. *P < 0.05; **P < 0.01; ***P < 0.001 determined by unpaired two-tailed Student t-test.