Figure 5
From: mTOR is critical for intestinal T-cell homeostasis and resistance to Citrobacter rodentium
Differential role of mTORC1 and mTORC2 in proliferative renewal of SI-LP and LI-LP T-cells.
(A) Death rates of SI-LP and LI-LP T-cells in mTORf/f-CD4Cre, Rptorf/f-CD4Cre, Rictorf/f-CD4Cre, and their corresponding control mice determined by staining with a LIVE/DEAD® Fixable Dead Cell Stain. (B–G) BrdU incorporation in SI-LP and LI-LP T-cells. mTORf/f-CD4Cre and mTORf/f mice (B,C), Rptorf/f-CD4Cre and Rptorf/f mice (D,E), and Rictorf/f-CD4Cre and Rictorf/f mice (F,G) were intraperitoneally (i.p) injected with BrdU and BrdU incorporation in SI-LP and LI-LP T-cells were examined by flow-cytometry 16 hours later. (B,D,F) Representative dot-plots showing BrdU staining in gated SI-LP and LI-LP CD4 and CD8 T-cells. (C,E,G) Scatter plots showing percentages of BrdU+ T-cells in the indicated populations. Horizontal bars represent the mean ± SEM. Each circle represents one mouse of the indicated genotypes. Data shown represent or calculated from at least three experiments. *P < 0.05; **P < 0.01; ***P < 0.001 determined by paired (E) and unpaired (others) two-tailed Student t-test.
