Figure 6

Assessment of mTORC1 in SI-LP and LI-LP T-cell proliferative renewal after acute mTORC1 ablation in mature T-cells.

T-cells isolated from CD45.2⁺Rptor^f/f-CreER and CD45.1⁺ WT mice were intravenously injected as a mixture into Rag2^−/− mice. One month later, recipient mice were i.p. injected with tamoxifen or PBS on days 1, 2, 5, and 12, and with BrdU on day 14. Mice were euthanized on day 15 for assessment of BrdU incorporation. Shown are: (A) Representative dot-plots of BrdU staining in SI-LP and LI-LP CD4 and CD8 T-cell subsets in gated CD45.1⁺TCRβ⁺ WT and CD45.2⁺TCRβ⁺ Rptor^f/f-CreER T-cells. (B) CD45.1 and CD45.2 expression in gated TCRβ⁺ SI-LP and LI-LP cells. Data shown are representative of two experiments.