Figure 2
Biological variance derived from FFPE samples representing ovarian carcinoma histotypes can be recapitulated in samples from different source materials.
Sets of 10 unique FFPE tumour samples (5 HGSC, 5 CCC) were prepared in biological duplicate using SP3-CTP. Alongside, a set of 8 (4 HGSC, 4 CCC) matched frozen tissue sections, and 6 cell lines (3 HGSC, 3 CCC) were prepared in biological duplicate and analyzed using the same pipeline. (a) Volcano plot depicting the variance in expression between the HGSC and CCC histotypes in the frozen tissue section data. Highlighted points indicate proteins identified from the 113-gene feature set previously shown to differentiate these ovarian carcinoma types. The dotted vertical lines indicate one standard deviation from the mean fold change. (b) Density smoothed scatter depicting the correlation of fold change values obtained from the FFPE and frozen tissue section data when comparing HGSC with CCC. (c) Volcano plot depicting the variance in expression between the HGSC and CCC histotypes in the cell line data. Highlighted points indicate proteins identified from the 113-gene feature set previously shown to diverge between these ovarian cancer types. The dotted vertical lines indicate one standard deviation from the mean fold change. (d) Density smoothed scatter depicting the correlation of fold change values obtained from the FFPE and cell line data when comparing HGSC with CCC. All correlation values were calculated using the Pearson method.
