Figure 7
Notch signaling activity is altered in ovaries of Gas2 null mice.
Paraffin embedded wild type and Gas2 null adult ovary sections were stained for Notch1 (brown). Increased intensity of Notch1 staining was observed in the ovarian stroma of Gas2 null mutant mice (A). The ability of GAS2 to modulate Notch signaling was examined in HeLa cells by luciferase assays using luciferase reporters driven by the promoters of Hes2 and Rbpj (B). The first two bars represent endogenous Notch signaling and the second two bars represent Notch signaling induced by a Notch1-ICD construct. Over-expression of GAS2 repressed both endogenous Notch signaling as well as Notch1-ICD induced signaling activity. (C) PCR arrays for Notch signaling pathway were performed on neonatal (P1) ovaries from wild type and Gas2 null mice. Genes with a more than a 2-fold difference in the levels of transcripts are shown. Genes with statistically different levels were highlighted in red (*p < 0.05; n = 4 for each genotype).
