Figure 4 | Scientific Reports

Figure 4

From: FOXA and master transcription factors recruit Mediator and Cohesin to the core transcriptional regulatory circuitry of cancer cells

Figure 4

Depletion of the core transcription factors phenocopies the loss of Mediator and Cohesin.

(a) Growth curve of MCF7, HEPG2 and A549 following decreased expression of ERa and FOXA1 in MCF7, HNF4A and FOXA2 in HEPG2 and FOLS2 and FOXA2 in A549. The results show a global negative effect on cell growth. Each line represents the average of a minimum of two biological replicates for two independent shRNA constructs with the exception of FOSL2 where one shRNA construct was used. The p-values were calculated by comparing with the shRNA controls at day 8 (paired t-test, *p < 0.05 and **p < 0.01). (b) Soft agar colony formation assays showing a loss of colony formation potential with decreased expression of core transcription factors. Colonies were quantified with the ImageJ software using 4 images per well. The bar graph represents the average of a minimum of two biological replicates for two independent shRNA constructs with the exception of FOSL2 where one shRNA construct was used. The p-values were calculated by comparing with the shRNA controls (paired t-test, *p < 0.05 and **p < 0.01).

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