Figure 4
From: SOXE neofunctionalization and elaboration of the neural crest during chordate evolution
Forced expression of AmphiSOXE induces neural crest-like cells in chick neural tube.
(a) Schematic representation of the experimental strategy. (b,f) Transverse sections of the neural tube electroporated with AmphiSoxE/EGFP or Sox9/EGFP analysed at 24 hours post-transfection. (c,g) Overexpression of AmphiSoxE or Sox9 induces ectopic HNK-1 expression. (e,i) Insets show a magnified view of the neural tube region with SOX2 negative cells shown by the white box. The neuronal progenitor marker SOX2 is repressed in (d,e) AmphiSOXE or (h,i) SOX9 expressing cells in a cell-autonomous manner. Scale bar: 50 μm.
