Figure 1
Establishment of a new protocol for Plasmodium falciparum sexual development in humanized mouse.
(a) Experimental procedure for engraftment of NSG mice with hRBC before infection with P. falciparum parasites. (b) Hematological parameters and mortality in 4 groups of mice (n = 4 mice per group) treated with different immunomodulation protocols. Protocol A: 33.3 mg/kg of lip-clod +10 mg/kg of mAb NIMP-R14, protocol B: 13.3 mg/kg of lip-clod +2 mg/kg of mAb NIMP-R14 and protocol C: 6.25 mg/kg of lip-clod +1 mg/kg of mAb NIMP-R14. Control mice received RPMI 1460 medium. Measurement of total RBC (mouse and human RBC, 106/mm3), platelets (PLT, 103/mm3), leucocytes (LCT, 103/mm3), hematocrit (HTC, %) and percentage of hRBC (hRBCpb) in mice peripheral blood samples were performed by using an automatic hematology analyzer at day 9 of the protocol. Mortality was recorded during 9 days and surviving mice (4 in Control group, 3 in group A, 2 in group B and 4 in group C) were sacrificed at day 9. Presence of hRBC in peritoneum (hRBCp) was determined after sacrifice. Complete coverage of this cavity with hRBC was noted as 2 points, partial coverage was noted as 1 point and absence of hRBC was noted as 0 point.
