Figure 4
From: Depletion of ribosomal protein S19 causes a reduction of rRNA synthesis
Protein analysis.
Total extracts from K562C cells untreated (−dox) or treated with doxycycline for 4 days to induce RPS19 depletion (+dox) or from 22Rv1 cells transfected with control (siCNT) or RPS19-specific siRNA (siS19) were analyzed by Western blot with the indicated antibodies. Examples of Western from a single experiment are shown in the upper panels. Quantification of signals from three independent experiments is reported in lower panels as a column plot of the mean ± s.e.m. of the densitometry values normalized by GAPDH. The image of total CDK2 in 22Rv1 cells was obtained by cropping two lanes from a single blot. Uncropped original images are shown in Supplementary information.
