Figure 5
Immunohistochemistry and western blotting of active caspase-3.
(a) Representative images of immunohistochemistry staining of cortex and hippocampus. Scale bar = 20 μm. (b) Bar graph shows a low positive rate of the sham group, the positive rate of the SAH + vehicle group were increased (*P < 0.01) compared with sham group, while the positive rate of SAH + methazolamide group was lower than the SAH + vehicle group (#P < 0.05). (c) Representative images of Western blot between different groups. (d) Western blot quantification showed that the sham group has no expression of active caspase-3. Expression of active caspase-3 in SAH + vehicle group increase obviously (*P < 0.01) and it decreased significantly in SAH + methazolamide group (#P < 0.05 vs SAH + vehicle group). N = 5 mice for each group.
