Figure 1

DNM2 expression in mouse testes and male germ cells.

(a) Fixed tissue sections from neonatal (D7-D18) and young adult (D28) mice testes were probed for DNM2 protein expression using immunofluorescence. The sections were counterstained with the nuclear marker DAPI and visualized using epifluorescence microscopy. (Scale bar = 50 μm). (b) Isolated SPG, PS and RS were fixed and DNM2 protein expression was examined using immunocytochemistry (Scale bar = 5 μm). (c) Representative immunoblots of DNM2 expression in whole testis from D7-D28. GAPDH was used as a loading control. (d) Representative immunoblots of DNM2 expression in isolated SPG, PS and RS compared to whole testis, with α-tubulin was used as a loading control. (e) qPCR analysis of Dnm2 in whole testis, SPG, PS and RS in C57Bl/6 males. Data are presented as mean relative expression to Cyclophilin A. (***p < 0.001, **p < 0.01 Student’s t-test ± SEM).