Figure 1

CCR9⁺Mφs are not pre-existing in steady state, but specifically accumulate to the injured liver.

Mice were intravenously injected with Con A (20 mg/kg), and CCR9 expression in each immune cell subset was sequentially analyzed by flow cytometry. (a) Sequential change in the frequency of CCR9⁺CD11b⁺ Mφs in each organ. Data show mean ± SEM (n = 3). BM; bone marrow, PB; peripheral blood, WAT; white epididymal adipose tissue, SI; small intestine, PP; Peyer’s patch, MLN; mesenteric lymph node. (b) Phenotipic characterization of CD11b⁺ cells in PBS-injected total liver mononuclear cells (left), Con A-injected total liver mononuclear cells (middle), and ConA-injected hepatic CCR9⁺CD11b⁺ gated cells (right). (c) Percentage of each surface marker negative (left) and positive (right) cells in Con A-injeted hepatic CCR9⁺CD11b⁺ gated cells. (d) Representative staining of CD11b and Ly6C on hepatic CCR9⁺CD11b⁺ gated cells at 6 hours (left) and 12 hours (right) following Con A injection. Data show mean ± SEM (n = 4). *p < 0.05, **p < 0.01.