Figure 4

CCR9⁺Mφs are originated from BM via blood circulating monocytes without contribution of hepatic resident Mφs.

Irradiated mice with a whole liver shield (shield) were reconstituted with BM cells. 6 weeks after BM transplantation, mice were further injected with Con A. (a) Left: Absolute cell numbers of total leukocytes in 1 ml PB of mice on 6 weeks after BMT. Right: The percentage of donor cells (chimerisms) in each cell fraction of PB. total monocytes: CD45⁺CD11b⁺Ly6G⁻Ly6C⁺⁺, Ly6C high or low monocytes: CD45⁺CD11b⁺Ly6G⁻Ly6C^{high or low}, granulocytes: CD45⁺CD11b⁺Ly6G⁺, plasmacytoid DC (pDC): CD45⁺CD11b⁻CD11c⁺. (b) Left: Correlation of the chimerism in monocytes fraction of PB and BM. Right: Correlation of the chimerism in monocytes fraction of PB and linage^-CD115⁺CD117⁺ MDP-like cells fraction of BM. (c) Left: Representative staining of CD45.1 (donor cells) and CD45.2 (recipient cells) on CD11b^lowF4/80^high resident Mφs and CD11b^highF4/80^low recruited Mφs in the liver of shielded BMT mice under steady state. Right: The percentage of donor-derived cells in resident Mφs and recruited Mφs. Data show mean ± SEM (n = 5). (d) Correlation between the chimerism of PB monocytes and the chimerism of recruited Mφs (white squares) or resident Mφs (black squares) in shielded BMT mice liver under steady state (n = 5 each) (e) Serum ALT levels of shielded BMT mice at 12 hours after Con A injection (inflammatory state). Data show mean ± SEM (n = 6). (f) Left: Correlation between the chimerism of PB monocytes and the chimerism of hepatic CD11b⁺CCR9⁺Mφs of shielded BMT mice under inflammatory state. Right: Representative CD11b and F4/80 staining on hepatic CD11b⁺CCR9⁺Mφs (blue dots) and whole hepatic cells (red dots). *p < 0.05, **p < 0.01. n.s.: not significant.