Figure 5

Recruited monocytes acquired CCR9 expression with local proliferation in the inflamed liver.

PBS or Con A injected mice were intraperitonealy administered with EdU solution (50 mg/kg) 2 hours before sacrifice. (a) Left: Representative CD11b and EdU staining on whole mononuclear cells in the liver (upper) and PB (lower) from PBS, PBS + EdU, or Con A + EdU treated mice. Right: Percentage of EdU⁺ cells in CD11b⁺ gated PB or liver mononuclear cells. Data show mean ± SEM (n = 3–4). (b) Left: Representative CCR9 and CD11b staining on EdU⁺ gated cells in the liver (upper) and PB (lower) Right: The percentage of CCR9⁺ cells in EdU⁺ gated cells in PB and the liver of mice in indicated groups. Data show mean ± SEM (n = 3–4). *p < 0.05, **p < 0.01.