Figure 4
Mitochondrial oxidative stress and apoptosis in the ovaries of mice following melatonin treatment for 12 mo.
(a): Mitochondrial ROS production. (b) The average telomere lengths of ovaries, shown as the T/S ratio, were determined by qRT-PCR. (c) Telomerase activities in the ovaries were analyzed by ELISA. (d) Indicators of mitochondrial DNA oxidation (8-OHdG). (e) GSSG/GSH ratio. Mitochondrial-related apoptosis were analyzed by western blotting. (f) Bax/Bcl-2 ratio. (g) Mitochondrial and cytosolic cytochrome c. Data are means ± SD (n = 7/group). *P < 0.05; **P < 0.01 versus the Young group. #p < 0.05; ##p < 0.01 versus the Vehicle group.
