Figure 6
Effect of melatonin on the H2O2-induced oxidative damage and apoptosis in granulosa cells in vitro.
(a) Cell viability was measured by CCK-8. (b) Mitochondrial reactive oxygen species (mROS). (c) GSSG/GSH ratio. (d) The elevation of mitochondrial oxidative stress was further confirmed by 8-OHdG. (e,f): Apoptosis rate. All values are the mean ± SD of the results from three independent experiments. **p < 0.01 versus the Control group. #p < 0.05; ##p < 0.01 versus the H2O2 group.
