Table 5 Effect of melatonin on pronuclear formation and development of MII oocytes after in vitro fertilization.
Length of treatment (mo) | Groups | No. of mice examined | No. of oocytes cultured | No. of oocytes forming pronuclei (%) | No. of embryo (%) | ||||
|---|---|---|---|---|---|---|---|---|---|
4 h | 5 h | 6 h | 7 h | 2-cell | Blastocysts | ||||
6 | Vehicle | 12 | 227 | 15(6.6)[7.4] | 76(33.5)[37.6] | 187(82.4)[92.6] | 202(89.0)[100] | 181(79.8) | 139(61.2) |
Melatonin | 12 | 235 | 16(6.8)[7.7] | 89(37.9)[42.6] | 197(83.8)[94.3] | 209(88.9)[100] | 190(80.9) | 149(63.4) | |
12 | Vehicle | 26 | 144 | 6(4.2)[5.1] | 51(35.4)[43.2] | 105(72.9)[89.0] | 118(81.9)[100] | 107(74.3) | 80(55.6) |
Melatonin | 19 | 186 | 21(11.3)*[13.5] | 94(50.5)**[60.3] | 150(80.6)*[96.2] | 156(83.9)[100] | 146(78.5)* | 117 (62.9)* | |
